Background: Acacia ataxacantha is a medicinal specie used extensively in traditional medicine of Benin republic to\ntreat infectious diseases. Our previous study showed interesting antibacterial and antifungal activities against six\nstrains of bacteria and six strains of fungi. The aim of this study was to investigate the antimicrobial and antioxidant\nactivities of compounds isolated from A. ataxacantha.\nMethods: Chromatographic and spectroscopic methods were used to isolate and identify three compounds (1ââ?¬â??3)\nfrom the bark of A. ataxacantha. Phytochemical investigation of A. ataxacantha (Fabaceae) led to the isolation of\nthree triterpenoids (1ââ?¬â??3). The structure of isolated compounds was established by differents spectroscopic methods\nsuch as UV, 1H NMR, 13C NMR, 2D NMR and Mass. All isolated compounds were tested for antimicrobial activity\nusing agar disc-diffusion and microdilution methods. The radical scavenging activity of isolated compounds was\nassessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method.\nResults: Phytochemical investigation led to the isolation and identification of lupeol (1), betulinic acid (2) and\nbetulinic acid-3-trans-caffeate (3). Moderate antimicrobial activity was obtained with compound 3 against\nmethicillin-resitant Staphylococcus aureus, Enterococcus feacalis and Pseudomonas aeruginosa with MIC value of\n25 Ã?¼g/ml and Staphylococcus aureus (MIC of 50 Ã?¼g/ml). Compounds 3 was more active against Staphylococcus\nepidermidis and Candida albicans with a MIC value of 12.5 Ã?¼g/ml in boths cases. Compounds 3 had also interesting\nantioxidant activity with an IC50 of 3.57 Ã?¼g/ml compared to quercetin (1.04 Ã?¼g/ml).\nConclusion: The overall results of this study provide evidence that the compound 3, isolated from A. ataxacantha,\nexhibit antimicrobial activity against Gram-positive and Gram-negative bacteria and yeast, especially against C.\nalbicans.
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